Abstract: As backward areas of social development and fragile ecological protection areas in China, most of the villages in frontier ethnic areas are not included in the traditional village list due to the lack of in-depth exploration of the unique value of villages. A few listed villages are scattered in space, and it is difficult to play a leading role in the process of rural revitalization. Facing the dilemma of traditional buildings and villages being destroyed and ecological resources deteriorating, the symbiosis between the local culture inheritance and the ecological sustainable development of villages in this region is an urgent problem to be solved. This paper sorts out and draws on the existing village evaluation system, takes Tumed region of Inner Mongolia as the research area, constructs the framework model of village ecological evaluation index system, uses SPSS24.0 analysis tool to refine the evaluation index factors comprehensively, and proposes a comprehensive evaluation system with ecological value of regional characteristic villages. Some villages with typical characteristics in the researched region are selected to verify the scientificity and applicability of the evaluation system, to provide reference ideas for the benign interaction and collaborative symbiosis between village development and ecology of Tumet area in Inner Mongolia and other frontier ethnic areas.