Abstract: The rural production organization mode has an important influence on the layout pattern of rural settlements. With the deepening of China's urbanization and the full scale implementation of new era rural vitalization strategy, great changes have taken place in rural production organization mode, as well as in urban and rural spatial layout. On the basis of analyzing the background of rural development in the new era, this article studies the changes of rural production organization mode, from the perspective of new-type business entities, newtype organization mechanism and operation mode changes, and then puts forward the conclusion that its influence on urban and rural spatial layout is spatial organization centralization, community-based residential form and public supporting facilities modernization.